Clone Assay:Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Luciferase:Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Plasmid Preparation:Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Sequencing:Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).
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