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backbone renilla luciferase vector pis2  (Addgene inc)


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    Structured Review

    Addgene inc backbone renilla luciferase vector pis2
    Backbone Renilla Luciferase Vector Pis2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pis2+luciferase+plasmid/pIS2+(Plasmid+%2312177)/pmc12529927-120-42-47
    Average 93 stars, based on 20 article reviews
    backbone renilla luciferase vector pis2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Luciferase:

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Plasmid Preparation:

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Sequencing:

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).

    Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
    Article Snippet: The resulting mutant plasmids were validated by sequencing, which was performed by Quintara Biosciences (Denver, CO, USA). .. LAMP1, cathepsin D 3′UTR and Josd1-ps sequences were cloned into a pIS2 luciferase plasmid (a gift from David Bartel, plasmid 12177; Addgene) and validated by sequencing. .. Mouse podocytes were transfected with 1 ug of pIS2 plasmid using ViaFect transfection reagent (Promega, Madison, WI, USA, cat# 4981, lot# 0000514926), and the cells were transfected with 50 nM of the miR control sequence or the miR-204-5p mimic or inhibitor sequences using the transfection reagent HiPerFect (Qiagen, Hilden, Germany, cat# 301705, lot# 172028439).



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    CXCL1 expression is inversely associated with TGF-β signaling in mammary fibroblasts. A . Conditioned medium from Flox/Flox or FspKO fibroblasts were analyzed for CXCL1 secretion by ELISA. B . Flox/Flox control or FspKO fibroblasts were co-transfected with CXCL1 firefly and <t>Renilla</t> <t>luciferase</t> reporter constructs and analyzed for luciferase activity. Values are normalized to Renilla. C .-D. Conditioned medium from carcinoma associated fibroblast cell lines (41CAF, 83CAF) or normal fibroblasts (NAF) were analyzed by ELISA for CXCL1 (C) or TGF-β secretion (D) . E . Fibroblasts were treated with 5 ng/ml TGF-β for 48 hours and analyzed for CXCL1 secretion by ELISA. Statistical analysis was determined by two-tailed Student t-tests. Statistical significance was determined by p <0.05. * p ≤0.001, ** p <0.05. Values are expressed as Mean ± SEM. Experiments were conducted in triplicate with three replicate samples per group.
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    CXCL1 expression is inversely associated with TGF-β signaling in mammary fibroblasts. A . Conditioned medium from Flox/Flox or FspKO fibroblasts were analyzed for CXCL1 secretion by ELISA. B . Flox/Flox control or FspKO fibroblasts were co-transfected with CXCL1 firefly and Renilla luciferase reporter constructs and analyzed for luciferase activity. Values are normalized to Renilla. C .-D. Conditioned medium from carcinoma associated fibroblast cell lines (41CAF, 83CAF) or normal fibroblasts (NAF) were analyzed by ELISA for CXCL1 (C) or TGF-β secretion (D) . E . Fibroblasts were treated with 5 ng/ml TGF-β for 48 hours and analyzed for CXCL1 secretion by ELISA. Statistical analysis was determined by two-tailed Student t-tests. Statistical significance was determined by p <0.05. * p ≤0.001, ** p <0.05. Values are expressed as Mean ± SEM. Experiments were conducted in triplicate with three replicate samples per group.

    Journal: BMC Cancer

    Article Title: Elevated CXCL1 expression in breast cancer stroma predicts poor prognosis and is inversely associated with expression of TGF-β signaling proteins

    doi: 10.1186/1471-2407-14-781

    Figure Lengend Snippet: CXCL1 expression is inversely associated with TGF-β signaling in mammary fibroblasts. A . Conditioned medium from Flox/Flox or FspKO fibroblasts were analyzed for CXCL1 secretion by ELISA. B . Flox/Flox control or FspKO fibroblasts were co-transfected with CXCL1 firefly and Renilla luciferase reporter constructs and analyzed for luciferase activity. Values are normalized to Renilla. C .-D. Conditioned medium from carcinoma associated fibroblast cell lines (41CAF, 83CAF) or normal fibroblasts (NAF) were analyzed by ELISA for CXCL1 (C) or TGF-β secretion (D) . E . Fibroblasts were treated with 5 ng/ml TGF-β for 48 hours and analyzed for CXCL1 secretion by ELISA. Statistical analysis was determined by two-tailed Student t-tests. Statistical significance was determined by p <0.05. * p ≤0.001, ** p <0.05. Values are expressed as Mean ± SEM. Experiments were conducted in triplicate with three replicate samples per group.

    Article Snippet: Cells were seeded in 6-cm dishes at a density of 150,000 cells for 24 hours, and then co-transfected with 8 μg of firefly luciferase plasmids (PGL3.luc.CXCL1) and 400 ng of Renilla luciferase plasmids (plasmid 12177: plS2, Addgene) using 8.4 μl Lipofectamine LTX and 15 μl Plus reagents according to manufacturer’s protocol (Invitrogen, life technologies).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Transfection, Luciferase, Construct, Activity Assay, Two Tailed Test